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目的 研究褐家鼠线粒体DNA细胞色素C氧化酶亚基I(COI)基因的遗传多态性特征,为国境口岸鼠形动物的鉴定和溯源提供技术支持。方法 通过对江苏口岸本底及输入性褐家鼠COI基因的扩增、测序和比对,结合Gen Bank的褐家鼠信息,对褐家鼠进行遗传多态性分析和系统发育树研究。结果 获得48个国内外褐家鼠COI基因序列,测出的605个序列位点中保守位点587个,可变异位点18个,核苷酸平均差异数为2.51000±0.00064;江苏口岸本底褐家鼠仅为1个单倍型,非江苏口岸褐家鼠多态性丰富。结论不同地区的褐家鼠具有一定的种内差异,该差异与地理位置分布有着一定的关系。
Objective To study the genetic polymorphism of mitochondrial DNA cytochrome C oxidase subunit I (COI) gene in Rattus norvegicus and provide technical support for the identification and tracing of the mouse-shaped animals at the border crossings. METHODS: The Rattus norvegicus was cloned and sequenced by comparing the COI gene of Rattus norvegicus with that of imported Rattus norvegicus in Jiangsu and the Rattus norvegicus Genbank. Results The COI gene sequences of 48 brown domestic rats were obtained. There were 587 conserved loci in 605 loci and 18 variable loci with the average number of nucleotide differences of 2.51000 ± 0.00064. The Jiangsu port background Rattus norvegicus is only 1 haplotype, non-Jiangsu border Rattus norvegicus rich in polymorphisms. Conclusion Rattus norvegicus has some intraspecies differences in different regions, which is related to the geographical distribution.