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利用Kinmaze(粳稻)/DV85(籼稻)杂交组合衍生的重组自交F11家系 (Recombinant Inbred Lines, RILs)进行了种子休眠性QTL的检测和遗传效应分析。以抽穗后35 d的种子发芽率作为种子休眠性的表型值,分析亲本和81个家系的休眠性表现,利用Windows QTL Cartographer 1.13a软件共检测到4个种子休眠性QTL,分别位于第2、5、11染色体上,其中第2染色体存在2个QTL,各QTL的贡献率变幅8.37%～17.40%。进一步研究了这些休眠性基因位点对干热破除休眠处理的响应,结果表明,来自DV85增强休眠性的QTL位点qDOR-2-1和qDOR-5,以及来自Kinmaze增强休眠性的QTL位点qDOR-11,易被干热处理破除休眠,这3个QTL效应较强,可在种子休眠性状的遗传改良中加以利用;而位于第2染色体上标记XNpb227-XNpb132之间的QTL位点qDOR-2-2却不易被干热处理破除休眠,该位点增强休眠性的基因来自DV85。
Seed dormancy QTLs and genetic effects were analyzed using recombinant inbred lines (RILs) derived from Kinmaze (japonica) / DV85 (indica) hybrid combinations. The seed dormancy of 35 days after heading was used as the seed dormancy phenotypic value to analyze the dormancy performance of the parents and 81 pedigrees. Four QTLs for dormancy were detected using Windows QTL Cartographer 1.13a software, There were two QTLs on chromosome 2 and chromosome 5, and the contribution rate of each QTL varied from 8.37% to 17.40%. Further studies on the response of these dormancy loci to dry-heat-disrupted dormancy treatments revealed that qDOR-2-1 and qDOR-5 QD loci from DV85 enhanced dormancy and QTL loci from enhanced Kinmaze dormancy QDOR-11, which was easily dry-heat-treated to break dormancy, had strong effect on the genetic improvement of seed dormancy traits. The QTL locus qDOR-2 located on chromosome 2 between XNpb227-XNpb132 -2 is not easy to get rid of dry heat treatment, the site of enhanced dormancy gene from DV85.