小麦 (TriticumaestivumL .)济南 177的两种原生质体 ,一种来自快速生长的悬浮细胞 ,它们因长期继代而丧失分化能力 ,其染色体只有 2n =2 4～ 2 8;另一种来自可以再生的愈伤组织 ,其原生质体不能持续分裂。它们中任一种与UV照射过的簇毛麦原生质体融合均不能再生植株。然而当它们混合在一起作为受体时 ,能够获得再生绿色植株。细胞核基因和胞质基因的分析证明这些绿色植株是杂种。以上事实说明这两种原生质体在融合时存在某些互补的关系 ,讨论了这种融合方式的可能作用及重要性。 Two protoplasts from Triticum aestivum L. Jinan 177, one from rapidly growing suspension cells, lose their ability to differentiate due to long-term succession, with chromosomes of 2n = 24 to 28 and the other from regenerable Callus, the protoplasts can not continue to divide. None of the plants fused with UV-irradiated clustergrass protoplasts regenerated the plants. However, when they are mixed together as a receptor, regenerated green plants can be obtained. Analysis of the nuclear and cytoplasmic genes proves that these green plants are hybrid. The above facts show that there is some complementary relationship between the two protoplasts during fusion, and the possible role and importance of this fusion method are discussed.