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目的研究慢性乙型肝炎病毒(HBV)感染者与健康者外周血B、T淋巴细胞亚群的差异,探讨外周血B、T淋巴细胞亚群的变化与HBV感染临床发生和发展的关系。方法选择2011年2月至2014年2月收治的门诊慢性HBV感染者78例,采用流式细胞仪和荧光定量聚合酶链反应法对78例慢性HBV感染者和50例健康体检者外周血B、T淋巴细胞亚群及HBV载量进行检测。结果健康体检组、HBV携带组和慢性乙肝组CD3+、CD3+CD4+、CD3+CD8+和CD4+/CD8+的细胞百分比逐渐递减,其中健康体检组与HBV携带组相比,差异无统计学意义(P>0.05),健康体检组、HBV携带组分别与慢性乙肝组相比,差异均有统计学意义(P<0.05);而3组CD19+、CD5+CD19+的细胞百分比值逐渐增高,其中健康体检组与HBV携带组相比,差异无统计学意义(P>0.05),健康体检组、HBV携带组分别与慢性乙肝组相比,差异有统计学意义(P<0.05);健康体检组与HBV DNA阴性组相比,外周血B、T淋巴细胞亚群各细胞百分比差异无统计学意义(P>0.05),健康体检组、HBV DNA阴性组与HBV DNA阳性组相比,差异均有统计学意义(P<0.05)。结论乙肝病毒感染可导致细胞免疫功能发生改变,外周血B、T淋巴细胞亚群的检测对判断乙肝病毒感染者病情进展有一定的临床意义。
Objective To study the difference of B and T lymphocyte subsets in peripheral blood of patients with chronic hepatitis B virus (HBV) infection and healthy subjects and to explore the relationship between the changes of B and T lymphocyte subsets in peripheral blood and clinical occurrence and development of HBV infection. Methods Seventy-eight outpatients with chronic HBV infection were selected from February 2011 to February 2014. Flow Cytometry and Fluorescent Quantitative Polymerase Chain Reaction were used to detect the levels of peripheral blood B in 78 chronic HBV infected patients and 50 healthy volunteers , T lymphocyte subsets and HBV load were detected. Results The percentage of CD3 +, CD3 + CD4 +, CD3 + CD8 + and CD4 + / CD8 + cells in healthy group, HBV-carrying group and chronic hepatitis B group decreased gradually. There was no significant difference between healthy group and HBV group (P> 0.05). There were significant differences in the levels of CD19 +, CD5 + CD19 + among the three groups (P <0.05). The percentage of cells in the three groups were increased gradually (P> 0.05). There was significant difference between healthy group and HBV-carrying group and chronic hepatitis B group (P <0.05). The difference between healthy group and HBV DNA-negative group was statistically significant There were no significant differences in the percentages of B and T lymphocyte subsets between healthy group and HBV DNA negative group as compared with HBV DNA positive group (P> 0.05) P <0.05). Conclusion Hepatitis B virus infection can lead to changes in cellular immune function. Detection of B and T lymphocyte subsets in peripheral blood may have certain clinical significance for judging the progression of hepatitis B virus infection.