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经眼眶入路行兔右侧大脑中动脉结扎(MCAO)造成实验性脑缺血,用原子吸收法测定局部脑组织Ca、Na和K的含量,用于湿重法计算局部脑组织含水量,发现在MCAO后3、5、8h,缺血区脑组织Ca、Na、H_2O积累,和非手术组比差异显著(P<0.01),在MCAO后5、8h,缺血区脑组织K丢失,和非手术组比差异亦显著(P<0.01)。Ca、Na积累大于K丢失导致缺血区脑组织内离子净增加,离子净增加和H_2O积累呈高度直线相关(r=0.8912,P<0.01)。结果提示:在兔MCAO 3、8h缺血区脑组织Ca和Na积累导致了缺血早期的脑水肿。
The experimental cerebral ischemia was induced by right middle cerebral artery occlusion (MCAO) in orbital approach. The contents of Ca, Na and K in local brain tissue were determined by atomic absorption spectrometry, and used to calculate the regional brain water content by wet weight method. It was found that there was significant difference in the accumulation of Ca, Na and H_2O in ischemic brain tissue at 3, 5, and 8 h after MCAO (P <0.01), and at 5 and 8 h after MCAO, The difference between the two groups was also significant (P <0.01). There was a high degree of linear correlation between the increase of Ca and Na over the K loss and the net increase of ions in the ischemic brain tissue. The net ion increase and H 2 O accumulation were highly linear (r = 0.8912, P <0.01). The results suggest that accumulation of Ca and Na in the ischemic brain of rabbits for 3 and 8 h resulted in cerebral edema in the early stage of ischemia.