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本实验采用ＡＢＣ一Ｄｏｔ—ＥＬＩＳＡ检测食品中单核白细胞增多症李氏杆菌。该法可检出在蛋白胨增菌液中接种的单核白细胞增多症李氏杆菌．其敏感性可达１０４个／ｍｌ，比Ｄｏｔ—ＥＬＩＳＡ约高１０—１００倍．特异性检测表明，其不与沙门氏菌、小肠结肠耶氏菌、蜡样芽胞杆菌、副溶血性弧菌发生交叉反应，但与葡萄菌和大肠杆菌有交叉反应．该法在２６ｈ内可报告结果，比常规法快３—５ｄ。对２３１份各种食品进行检测，检出４１份，经生化试验复检，３８份为单核白细胞增多症李氏杆菌，阳性率１６．４５％，假阳性率７．３２％，常规法的检出率１７．３１％。两种方法比较，结果相差不显著（Ｐ＞０．０５）。进行３次重复性试验，结果完全相同。实验结果表明，该法操作简便快速，具有较高的特异性和敏感性，可在２６ｈ报告结果，适于对大批样品进行快速检测，便于基层推广使用．
In this experiment, ABC-Dot-ELISA was used to detect Listeria monocytogenes in food. This method detects lentobacterium monocytogenes inoculated in peptone broth. Its sensitivity up to 104 / ml, about 10-100 times higher than Dot-ELISA. Specific tests showed that it did not cross-react with Salmonella, Enterocolitis, Bacillus cereus, Vibrio parahaemolyticus, but cross-reacted with Staphylococcus and Escherichia coli. The law can report the results within 26h, 3-5d faster than the conventional method. A total of 231 samples of various foods were detected and 41 were detected. After biochemical tests, 38 were Listeria monocytogenes, with a positive rate of 16.45% and a false positive rate of 7.32% The detection rate was 17.31%. There was no significant difference between the two methods (P> 0.05). Repeat the test three times, the results are exactly the same. The experimental results show that the method is simple and rapid, has high specificity and sensitivity, can report the results in 26h, suitable for rapid detection of a large number of samples for the promotion of grassroots use.