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目的:建立超高效液相色谱法(UPLC)同时测定人参、红参、西洋参中人参皂苷Rg1、Re、Rb1质量方法。方法:采用AcquityUPLCBEHC18色谱柱(2.1mm×100mm,1.7μm),以乙腈-水为流动相进行梯度洗脱,流速为0.5mL/min,检测波长为203nm,柱温为40℃。结果:人参、红参、西洋参中人参皂苷Rg1、Re、Rb1在14min内得到完全分离并进行了质量测定。结论:应用UPLC法能够同时测定人参、红参、西洋参中人参皂苷Rg1、Re、Rb1质量,大大提高了人参皂苷类成分的分析速度,减少了溶剂消耗,为人参皂苷类成分质量测定提供了参考。
Objective: To establish a method for simultaneous determination of ginsenoside Rg1, Re, Rb1 in ginseng, red ginseng and American ginseng by ultra performance liquid chromatography (UPLC). Methods: The mobile phase was eluted with acetonitrile using acetonitrile as the mobile phase. The flow rate was 0.5 mL / min and the detection wavelength was 203 nm. The column temperature was 40 ℃. Results: Ginsenoside Rg1, Re, Rb1 in ginseng, red ginseng and American ginseng were completely separated within 14 min and their quality was determined. CONCLUSION: UPLC method can simultaneously determine the quality of ginsenoside Rg1, Re, Rb1 in ginseng, red ginseng and American ginseng, greatly improve the analysis speed of ginsenosides and reduce the solvent consumption, which provides a reference for the determination of ginsenoside constituents. .