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目的 获得中国IL - 16cDNA ,供体外表达和转染使用。方法 从组胺刺激的正常人外周血单核细胞中分离总RNA ,利用半巢式RT -PCR、T -A克隆等技术得到特异的片段cDNA。结果 序列测定证实 ,所得片断确实为IL - 16cDNA。结论 成功克隆了中国人白细胞介素IL - 16cDNA。
Objective To obtain IL - 16 cDNA from China for in vitro expression and transfection. Methods Total RNA was isolated from normal human peripheral blood mononuclear cells stimulated by histamine, and specific cDNA fragments were obtained by semi-nested RT-PCR and T-A cloning techniques. Results sequencing confirmed that the fragment was indeed IL - 16 cDNA. Conclusion The Chinese IL - 16 cDNA was successfully cloned.