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在氨基甲酸乙酯麻醉、制动的大鼠 ,研究尾端延髓腹外侧区 (CVLM)中 NMDA和非 NMDA受体在介导动脉压力感受器反射 (ABR)中的作用。双侧 CVLM微量注射选择性 NMDA受体拮抗剂氯胺酮 (50mmol/L ,1 0 0 nl)或非 NMDA受体拮抗剂 kynurenic acid(KYA,50 mmol/L ,1 0 0 nl)后平均动脉压 (MAP)和心率 (HR)均明显升高 (P<0 .0 5) ,同时观察到 CVLM微量注射氯胺酮或 KYA后电刺激主动脉神经(AN)导致的血压下降比对照有明显的减少 ,双侧 CVLM微量注射氯胺酮和 KYA的混合物 (均为 50mmol/L,50 nl)后能完全阻断电刺激 AN后导致的降压反应。本研究结果提示 CVLM中 NMDA和非 NM-DA受体在紧张性维持交感神经的兴奋活动和介导 ABR中起十分重要的作用
In a urethane anesthetized, braked rat, the role of NMDA and non-NMDA receptors in mediating arterial baroreceptor reflex (ABR) was studied in the caudal venule rostral ventrolateral medulla (CVLM). Bilateral CVLM microinjection of selective NMDA receptor antagonist Ketamine (50 mmol / L, 100 nl) or non-NMDA receptor antagonist kynurenic acid (KYA, 50 mmol / L, 100 nl) mean arterial pressure MAP and HR (P <0.05). At the same time, the decrease of blood pressure induced by electrical stimulation of aortic nerves (AN) with CVLM after ketamine or KYA injection was significantly decreased compared with the control Side CVLM microinjection of a mixture of ketamine and KYA (both 50 mmol / L, 50 nl) completely blocked the antihypertensive response to electrical stimulation of AN. Our results suggest that NMDA and non-NMDA receptors in CVLM play a very important role in the tension-maintaining sympathetic activity and mediating ABR