论文部分内容阅读
详细介绍了椰毒假单胞菌部分１６ｓｒＲＮＡ序列测定法。常规提取细菌ＤＮＡ，采用聚合酶链反应（ＰＣＲ）扩增目的基因，琼脂糖凝胶电泳后回收目的基因。纯化后克隆到Ｍ１３ｍｐ１９噬菌体上，转染大肠杆菌ＪＭ１０９菌株。挑选无色噬斑扩增并提取其双链ＤＮＡ，酶切分析为阳性克隆，提取其单链ＤＮＡ在ＡＢＩ公司３７０Ａ型自动测序仪上测序。
The 16s rRNA sequence of Pseudomonas solanacearum is described in detail. Bacterial DNA was routinely extracted, and the target gene was amplified by polymerase chain reaction (PCR). The target gene was recovered after agarose gel electrophoresis. After purification, it was cloned into M13mp19 phage and transfected into Escherichia coli JM109 strain. The colorless plaques were selected for amplification and extraction of the double-stranded DNA. The positive clones were analyzed by restriction enzyme digestion and single-stranded DNA was extracted and sequenced on an ABI 370A automatic sequencer.