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目的:建立一种检测HIV-1特异性CD4n +T细胞亚群功能的活化诱导标记法(activation-induced markers，AIM)，从而更有效地评价HIV-1抗原特异性CD4n +T细胞免疫应答水平。n 方法:选取12例未经抗病毒治疗的HIV-1慢性感染者及6例未感染HIV-1的健康人，分别以基于多色流式细胞术的AIM法和细胞内因子染色法(intracellular cytokine staining，ICS)检测抗原特异性T淋巴细胞功能，并探讨两种方法用于评价HIV-1感染者抗原特异性T细胞免疫应答的能力。结果:AIM法检测HIV-1慢性感染者中HIV-1抗原特异性PD-1n +CD25n +CD4n +T、CD69n +CD200n +CD4n +T、CD69n +ICOSn + CD4n +T细胞阳性的比例为11/12、8/12和7/12，检测CD69n +ICOSn +CD8n +T、CD137n +CD69n +CD8n +T、PD-1n +CD25n +CD8n +、OX40n +PD-1n +CD8n +T细胞阳性的比例为8/12、8/12、7/12、7/12。ICS法检测HIV-1抗原特异性IL-2n +CD4n +T、IFN-γn +CD4n +T、TNF-αn +CD4n +T细胞阳性的占比为2/12、2/12、0；IFN-γn +CD8n +T、TNF-αn +CD8n +T、IL-2n +CD8n +T细胞阳性的占比为12/12、10/12、5/12。n 结论:AIM法在评价CD4n +T细胞功能方面更为敏感，可作为ICS法的补充，两种方法联合使用可更全面地评估抗原特异性T淋巴细胞反应。n “,”Objective:To establish a method using activation-induced markers (AIM) to detect the function of HIV-1-specific CD4n + T cell subsets for evaluating the immune response of HIV-1-specific CD4n + T cells more effectively.n Methods:Twelve chronically HIV-1-infected patients without antiviral therapy and six healthy people without HIV-1 infection were enrolled in this study. The function of HIV-1-specific T lymphocytes was detected by AIM and ICS based on polychromatic flow cytometry. The performance of the two methods in assessing HIV-1-specific CD4n + T cell immune response in HIV-1-infected patients was evaluated.n Results:The positive rates of HIV-1-specific PD-1n + CD25n + CD4n + T, CD69n + CD200n + CD4n + T, CD69n + ICOSn + CD4n + T, CD69n + ICOSn + CD8n + T、CD137n + CD69n + CD8n + T、PD-1n + CD25n + CD8n + T and OX40n + PD-1n + CD8n + T cells in all of the HIV-1 patients were 11/12, 8/12, 7/12, 8/12, 8/12, 7/12 and 7/12 using AIM method. ICS results showed that the positive rates of HIV-1-specific IL-2n + CD4n + T, IFN-γn + CD4n + T, TNF-αn + CD4n + T, IFN-γn + CD8n + T, TNF-αn + CD8n + T and IL-2n + CD8n + T cells were 2/12, 2/12, 0, 12/12, 10/12 and 5/12, respectively.n Conclusions:AIM method was more sensitive in antigen-specific CD4n + T cell detection, and could be used as a complementary method to ICS in assessing antigen-specific T cell response.n