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目的建立复方玄参止咳胶囊中哈巴俄苷的测定方法。方法采用高效液相色谱法,Irregular C18(250mm×4.6mm,10μm);乙腈-1.0%醋酸(26∶74)为流动相;检测波长为278nm;柱温为室温。结果哈巴俄苷在0.061～1.076μg线性关系良好,r=0.9999,平均加样回收率为99.6%,RSD为2.06%。结论该方法简便、快速、准确,为复方中测定哈巴俄苷提供了可靠的方法。
Objective To establish a method for determination of harpagoside in compound Xuan Shen Zhi Ke capsule. Methods High performance liquid chromatography was used. Irregular C18(250mm×4.6mm, 10μm); acetonitrile-1.0% acetic acid(26∶74) as mobile phase; detection wavelength was 278nm; column temperature was room temperature. Results The linear relationship of harpagoside was good in 0.061～1.076μg, r=0.9999, the average recovery was 99.6%, RSD was 2.06%. Conclusion This method is simple, rapid and accurate, and provides a reliable method for the determination of harpagoside in the compound.