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目的:探讨17β-雌二醇对人根尖牙乳头干细胞(stem cells from apical papilla,SCAPs)核因子-κB受体活化因子配体(receptor activator for nuclear factor-κB ligand,RANKL)、骨保护素(osteoprotegerin,OPG)表达的影响。方法:通过酶消化法分离培养SCAPs,将SCAPs分别于对照组(普通培养基+10μmol/L无水乙醇)和含0.1μmol/L17β-雌二醇的培养基培养3d和7d后,实时荧光定量PCR法和蛋白质免疫印迹法分别检测RANKL、OPG mRNA和蛋白表达。结果:17β-雌二醇刺激3d和7d组,OPGmRNA和蛋白表达均较对照组升高(P<0.01),RANKL mRNA和蛋白表达均较对照组降低(P<0.01)。结论:17β-雌二醇可能通过调节RANKL/OPG系统,参与调节SCAPs成牙/骨及破牙/骨活性。
Objective: To investigate the effect of 17β-estradiol on the expression of receptor activator for nuclear factor-κB ligand (RANKL), osteoprotegerin (osteoprotegerin, OPG) expression. Methods: SCAPs were isolated and cultured by enzymatic digestion. SCAPs were cultured in the control group (normal medium + 10μmol / L absolute ethanol) and the medium containing 0.1μmol / L 17β-estradiol for 3d and 7d, respectively. Real-time fluorescence quantitative PCR and Western blotting were used to detect RANKL, OPG mRNA and protein expression respectively. Results: Compared with the control group, the mRNA and protein expressions of OPG increased (P <0.01) and the expressions of OPG mRNA and protein in 3d and 7d groups decreased (P <0.01). CONCLUSION: 17β-estradiol may be involved in the regulation of tooth / bone and tooth decay / osteogenesis activity of SCAPs through the regulation of RANKL / OPG system.