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目的研究Neuregulin-1β对容量超负荷所致心衰大鼠的治疗作用。方法 34只SD大鼠随机分成心衰组、治疗组、假手术组。心衰组和治疗组采用腹主动脉-腔静脉穿刺造瘘法制备模型。假手术组只行主动脉穿刺不造瘘。治疗组造瘘术后8周给予Neuregulin-1β尾静脉注射,10μg/(kg.d),共7d。心衰组和假手术组给予同等剂量的生理盐水。12周后分别采用心动超声、血流动力学、血浆BNP浓度评价心功能;行病理学检查及观察心肌组织的超微结构。结果①心动超声显示治疗组左室射血分数、短轴缩短率高于心衰组(P<0.05),左室收缩末内径、舒张末内经小于心衰组(P<0.05)。②血流动力学检测显示治疗组左室收缩末压和左室内压最大上升、下降速率均明显高于心衰组(P<0.05);左室舒张末压低于心衰组(P<0.05)。③治疗组血BNP水平明显低于心衰组(P<0.01)。④与心衰组比较,治疗组光镜及透射电镜下心肌细胞受损程度明显减轻。结论 Neuregulin-1β可明显改善容量超负荷心衰大鼠的心功能、减轻心肌细胞的损伤、纠正心衰。
Objective To study the therapeutic effect of Neuregulin-1β on heart failure rats induced by volume overload. Methods Thirty-four SD rats were randomly divided into heart failure group, treatment group and sham operation group. Heart failure group and treatment group using abdominal aorto-vena cavaostomy model. Sham-operated group only aortic puncture did not make fistula. Neuregulin-1β was given to the tail vein of the treatment group at 8 weeks after the ostomy, at 10 μg / (kg · d) for 7 days. Heart failure group and sham operation group were given the same dose of saline. Twelve weeks later, cardiac function was evaluated by echocardiography, hemodynamics and plasma BNP concentration respectively. Pathological examination was performed to observe the ultrastructure of myocardium. Results ① The left ventricular ejection fraction and the short axis shortening rate in the treatment group were significantly higher than those in the heart failure group (P <0.05). The mean LV end systolic diameter and end diastolic diameter were lower than those in the HF group (P <0.05). (2) Hemodynamic examination showed that left ventricular end-systolic pressure and left ventricular pressure increased and decreased significantly in treatment group (P <0.05), left ventricular end-diastolic pressure was lower than that in HF group (P <0.05) . ③ The blood BNP level in the treatment group was significantly lower than that in the HF group (P <0.01). ④Compared with the group of heart failure, the damage of myocardial cells in the treatment group under light microscope and transmission electron microscope was significantly reduced. Conclusion Neuregulin-1β can significantly improve cardiac function, reduce the damage of myocardial cells and correct heart failure in rats with volume overload and heart failure.