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目的探讨外周血T淋巴细胞HLA-B27和血清多项免疫指标检测对强直性脊柱炎(AS)的辅助诊断价值。方法采用流式细胞仪对52例疑似AS患者外周血T淋巴细胞HLA-B27表达进行检测,结合临床资料对AS作出诊断;对其中37例男性病例血清免疫球蛋白IgG、IgA、IgM,补体C3、C4及C反应蛋白(CRP)含量进行免疫比浊法测定。结果52例疑似AS患者共检测出HLA-B27+13例(男性12例,女性1例),确诊AS患者12人(男性患者11人,女性1人),包括男性HLA-B27+AS患者9例(最小年龄12岁,最大58岁),男性HLA-B27-AS2例(分别为20岁和78岁),女性HLA-B27-AS1例为(9岁),男性AS确诊患者的HLA-B27阳性率为82%。除补体C3外,男性AS组IgG、IgA、IgM、C4及CRP均较男性非AS组高(P<0.05)。结论AS患者体液免疫功能明显活跃,HLA-B27检测有助于临床资料疑似病例的AS诊断。
Objective To investigate the diagnostic value of multiple HLA-B27 and serum immune markers in peripheral blood T helper lymphocytes in the diagnosis of ankylosing spondylitis (AS). Methods Flow cytometry was used to detect the expression of HLA-B27 in peripheral blood T lymphocytes of 52 patients with suspected AS. According to the clinical data, AS was diagnosed. Serum immunoglobulin IgG, IgA, IgM, complement C3 , C4 and C-reactive protein (CRP) were measured by immunoturbidimetry. Results A total of 52 cases of suspected AS patients were detected HLA-B27 13 cases (12 males and 1 females), 12 patients diagnosed with AS (11 males and 1 females), including male HLA-B27 + AS patients 9 Cases of HLA-B27-AS2 (20 and 78 years old, respectively) in men, HLA-B27-AS1 in women (9 years) and HLA-B27 in men diagnosed as AS The positive rate was 82%. Except for complement C3, the levels of IgG, IgA, IgM, C4 and CRP in AS group were significantly higher than those in non-AS group (P <0.05). Conclusion The humoral immune function of patients with AS is obviously active. The detection of HLA-B27 is helpful for the diagnosis of AS in suspected cases of clinical data.