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目的研究FOXA2在胃腺癌中的表达及其与胃癌细胞侵袭迁移能力的相关性。方法收集56例胃腺癌组织及对应的癌旁组织,运用免疫组织化学技术检测FOXA2和上皮型钙黏素(E-cadherin)在胃腺癌及对应癌旁组织中的表达;Western blot法检测FOXA2和E-cadherin蛋白在不同胃癌细胞株中的表达水平;在MKN-45胃癌细胞中过表达FOXA2,TranswellTM法检测胃癌细胞迁移和侵袭能力。采用Spearman法进行相关性分析。结果 FOXA2和E-cadherin蛋白在胃腺癌组织中表达水平显著低于对应癌旁组织;胃腺癌组织中FOXA2与E-cadherin蛋白表达呈显著正相关;FOXA2蛋白表达与肿瘤分化、浸润深度、淋巴结转移和TNM分期具有显著的相关性;高侵袭性MKN-45胃癌细胞中FOXA2和E-cadherin表达最低,而低侵袭性N-87胃癌细胞中两者表达最高;FOXA2过表达显著上调E-cadherin蛋白表达,且明显抑制MKN-45细胞迁移和侵袭能力。结论 FOXA2在胃腺癌组织中表达降低,其低表达与胃腺癌的恶性临床病理特征相关。FOXA2过表达上调E-cadherin表达并抑制胃癌细胞侵袭、迁移能力。
Objective To investigate the expression of FOXA2 in gastric adenocarcinoma and its correlation with invasion and migration of gastric cancer cells. Methods Fifty-six gastric adenocarcinoma tissues and corresponding paracancerous tissues were collected. The expression of FOXA2 and E-cadherin in gastric adenocarcinoma and corresponding paracancerous tissues was detected by immunohistochemistry. The expressions of FOXA2 and E-cadherin protein expression in different gastric cancer cell lines; overexpression of FOXA2 in MKN-45 gastric cancer cells, TranswellTM detection of gastric cancer cell migration and invasion. Spearman method was used for correlation analysis. Results The expression of FOXA2 and E-cadherin in gastric adenocarcinoma was significantly lower than that in paracancerous tissues. There was a significant positive correlation between the expression of FOXA2 and E-cadherin in gastric adenocarcinoma. The expression of FOXA2 was correlated with tumor differentiation, depth of invasion, lymph node metastasis And TNM staging. FOXA2 and E-cadherin were the lowest in highly invasive MKN-45 gastric cancer cells, while the lowest in invasive N-87 gastric cancer cells, FOXA2 overexpression significantly up-regulated E-cadherin protein Expression, and significantly inhibited MKN-45 cell migration and invasion. Conclusions The expression of FOXA2 in gastric adenocarcinoma is decreased, and its low expression is correlated with the clinicopathological features of gastric adenocarcinoma. Overexpression of FOXA2 upregulates E-cadherin expression and inhibits gastric cancer cell invasion and migration.