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目的从细胞调亡角度探讨VP16抗肿瘤作用的机理。方法用光学显微镜及电子显微镜观察了VP16诱导Raji细胞凋亡的形态学变化；用琼脂糖凝胶电泳观察了DNA的梯形改变。结果VP16可明显诱导Raji细胞凋亡。5μg·ml-1VP16作用48h时，凋亡指数为58．33％±3．06％，与对照组相比有显著性差异（P＜0．01）。调亡指数与药物浓度的对数呈正相关（r＝0．9407，P＜0．01）。结论诱导凋亡是VP16抗肿瘤作用的机理之一。
Objective To explore the anti-tumor mechanism of VP16 from the perspective of apoptosis. Methods The morphological changes of apoptosis induced by VP16 in Raji cells were observed by light microscope and electron microscope. The trapezoidal changes of DNA were observed by agarose gel electrophoresis. Results VP16 significantly induced apoptosis in Raji cells. The apoptosis index was 58.33%±3.06% when treated with 5μg·ml-1VP16 for 48h, which was significantly different from the control group (P<0.01). Apoptotic index was positively correlated with the logarithm of drug concentration (r=0.9407, P<0.01). Conclusion Induction of apoptosis is one of the mechanisms of VP16 antitumor effect.