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为鉴定蓝舌病病毒(BTV)的单克隆抗体(MAb)所识别的VP2蛋白竞争抑制型特异性抗原表位,本研究采用噬菌体展示技术对BTV VP2蛋白抗原表位氨基酸进行筛选。经过3轮淘选后进行测序,测序结果经分析比对后获得共同的短肽序列为~(160)NH~(161)。~(160)NH~(161)与已有的单抗杂交瘤细胞4A-1G7上清、腹水均发生特异性反应,并且~(160)NH~(161)与4型BTV阳性标准血清反应良好。本研究结果为4型BTV检测方法的建立及VP2蛋白结构和功能的研究奠定了基础。
In order to identify VP2 protein-specific inhibitory epitopes recognized by the monoclonal antibody (MAb) of the blue tongue virus (BTV), phage display technology was used to screen the amino acid of epitope of BTV VP2 protein. After 3 rounds of panning, sequencing was carried out. The common short peptide sequence was ~ (160) NH ~ (161) after analysis and comparison. ~ (160) NH ~ (161) reacted specifically with the McAb 4A-1G7 supernatant and ascites fluid, and ~ (160) NH ~ (161) reacted well with serotype 4 BTV positive serum . The results of this study laid the foundation for the establishment of the 4-type BTV detection method and the study of VP2 protein structure and function.