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目的 :探讨 p27mRNA与蛋白在成骨性肿瘤中的表达状况 ,了解其与肿瘤组织学类型、分化程度及生物学行为的关系。方法 :采用免疫组化和非放射性核酸原位杂交技术检测成骨性肿瘤中 p27蛋白和mRNA的表达状况。结果 :骨瘤、骨母细胞瘤中 p27蛋白高表达 ,主要定位于胞核 (85.7 %、75 % ) ,骨肉瘤中低表达 ,胞核表达极低(4.7 % ) ,两者间差异显著 (P<0.01)。p27蛋白在骨肉瘤中的表达与肿瘤的浸润、复发和转移有关。原位杂交显示良、恶性成骨性肿瘤的 p27mRNA表达阳性率及表达强度无显著性差异 (P>0.05)。且与骨肉瘤的组织学类型、分级及生物学行为无关。结论 :p27蛋白表达在成骨性肿瘤中的改变具有特异性 ,可作为骨肉瘤的诊断和评估预后的指标。骨肉瘤中p27蛋白表达低下可能是由于转录后特异性的蛋白酶介导的降解所致。
OBJECTIVE: To investigate the expression of p27 mRNA and protein in osteogenic tumors and its relationship with tumor histological type, differentiation and biological behavior. Methods: Immunohistochemistry and non-radioactive in situ hybridization were used to detect the expression of p27 protein and mRNA in osteogenic tumors. Results: The expression of p27 protein in osteoma and osteoblastoma was mainly located in the nucleus (85.7%, 75%), low expression in osteosarcoma (4.7%), and the difference was significant P <0.01). The expression of p27 protein in osteosarcoma is related to tumor invasion, recurrence and metastasis. In situ hybridization showed that there was no significant difference in the positive rate and expression intensity of p27mRNA between benign and malignant osteogenic tumors (P> 0.05). And with the histological type of osteosarcoma, grading and biological behavior has nothing to do. Conclusion: The expression of p27 protein in osteosarcoma is specific and can be used as a diagnostic and prognostic indicator for osteosarcoma. The low expression of p27 protein in osteosarcoma may be due to post-transcriptional specific protease-mediated degradation.