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目的:观察含金属硫蛋白(MT)蛋奶粉对BALB/c小鼠H-22肝癌的预防作用。方法:雌性BALB/c纯系小鼠70只随机分为正常对照组(10只)、荷瘤鼠对照组(20只)、含MT蛋奶粉预防肿瘤低剂量及高剂量组(各20只)。将MT低和高剂量蛋奶粉给小鼠灌胃2周后,皮下接种H-22细胞,建立小鼠移植肿瘤模型,观察MT蛋奶粉对肿瘤出现时间及肿瘤大小和生长的影响;观察1个月后处死小鼠,MTT法检测脾脏淋巴细胞转化功能,流式细胞术检测脾脏CD4+、CD8+和CD25+T细胞百分率以及细胞周期进程和凋亡的变化,3H-TdR掺入法检测CTL和NK细胞杀伤活性。结果:与荷瘤对照组相比,含MT蛋奶粉组小鼠其肿瘤生长速率显著降低(P<0.05),淋巴细胞增殖率显著增加(P<0.01),脾脏CD4+和CD8+T细胞百分率及CD4+/CD8+比值显著升高(P<0.05或P<0.01),CD25+T细胞百分率和淋巴细胞凋亡百分率均显著降低(P<0.05或P<0.01),NK细胞毒性显著提高(P<0.05或P<0.01),CTL细胞毒性亦显著增高(P<0.01)。结论:含MT蛋奶粉可明显增强荷瘤小鼠的免疫功能,对小鼠移植性肿瘤的生长起到一定程度的抑制作用。
Objective: To observe the preventive effect of metallothionein (MT) milk powder on H-22 hepatocarcinoma in BALB / c mice. Methods: Seventy female BALB / c mice were randomly divided into normal control group (n = 10), control group (n = 20) and control group (n = 20) . The mice were inoculated with low and high dose of powdered milk of multivitamin for 2 weeks. H-22 cells were inoculated subcutaneously to establish the model of mouse transplanted tumor. The effect of MT milk powder on tumor appearance and tumor size and growth was observed. One Mice were sacrificed after a month, the function of spleen lymphocyte transformation was detected by MTT assay, the percentage of CD4 +, CD8 + and CD25 + T cells and the cell cycle progression and apoptosis were detected by flow cytometry. 3H-TdR incorporation assay was used to detect CTL and NK Cell killing activity. Results: Compared with the tumor-bearing control group, the mice with MT milk powder group had significantly lower tumor growth rate (P <0.05), lymphocyte proliferation rate (P <0.01), percentage of spleen CD4 + and CD8 + T cells and (P <0.05 or P <0.01). The percentages of CD25 + T cells and lymphocyte apoptosis were significantly decreased (P <0.05 or P <0.01), and the cytotoxicity of NK cells was significantly increased (P <0.05 or P <0.01) Or P <0.01), CTL cytotoxicity was also significantly increased (P <0.01). CONCLUSION: Milk powder containing MT can obviously enhance the immune function of tumor-bearing mice and inhibit the growth of transplanted tumors in mice to a certain extent.