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以鲑鱼基因组ＤＮＡ为模板，采用ＰＣＲ获得ｓＣＴ基因，并为ＤＮＡ序列分析所证实．以ｐＧＥＸ－３Ｘ为表达载体，利用体外定点突变技术成功地构建了融合蛋白ＧＳＴ－ｓＣＴ的重组表达质粒ｐＧＥＸ－３Ｘ－ｓＣＴ，在大肠杆菌中得到高效表达，其表达量约为菌体总蛋白的３０％；利用亲合层析法对融合蛋白ＧＳＴ－ｓＣＴ进行纯化，再经Ｆａｃ－ｔｏｒΧａ酶切后获得了重组ｓＣＴ，并对其进行活性检测．初步实验证明，重组ｓＣＴ具有较强的生物活性
The sCT gene was obtained by PCR using salmon genomic DNA as a template and confirmed by DNA sequence analysis. The recombinant plasmid pGEX-3X-sCT was successfully constructed using pGEX-3X as the expression vector and the recombinant plasmid pGEX-3X-sCT of fusion protein GST-sCT was successfully constructed. The recombinant plasmid pGEX-3X-sCT was highly expressed in E. coli. 30%. The fusion protein GST-sCT was purified by affinity chromatography, then the recombinant sCT was obtained after digestion with Fac-torΧa, and its activity was detected. Preliminary experiments show that recombinant sCT has strong biological activity