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首次采用滤纸扩散法和平板涂布法对木蹄层孔菌Fomes fomentarius(L.:Fr.)Kick.子实体的水、甲醇、丙酮、乙酸乙酯、氯仿、石油醚等提取物进行了抑菌活性研究。结果表明,木蹄层孔菌各提取物对白色念珠菌JLC31680、白色念珠菌JLC31681、大肠杆菌ATCC 8099、金黄色葡萄球菌ATCC 6538均有不同程度的抑制作用。其中水提物与其他5个提取物相比,对金黄色葡萄球菌ATCC 6538的抑制作用最强,其次对大肠杆菌ATCC 8099,当浓度为100 mg.mL-1时,抑制率分别为95.54%和73.80%,最小抑菌浓度(MIC)分别是50 mg.mL-1、25 mg.mL-1。对白色念珠菌JLC31680和白色念珠菌JLC31681的抑制作用也较为明显,当浓度为100 mg.mL-1时,抑菌率分别为33.06%和26.67%,最小抑菌浓度(MIC)分别是12.5 mg.mL-1、50 mg.mL-1。甲醇提取物与其他5个提取物相比,对大肠杆菌ATCC 8099和金黄色葡萄球菌ATCC 6538的抑制作用最强,当浓度为50 mg.mL-1时,抑菌率分别为99.39%和95.10%,最小抑菌浓度(MIC)是12.5 mg.mL-1、25 mg.mL-1,且均有量效关系。
For the first time, the extracts of water, methanol, acetone, ethyl acetate, chloroform, petroleum ether and other extracts of Fomes fomentarius (L.:Fr.) Kick. Bacteria activity research. The results showed that the extract of Mycophorum pleuropneumoniae Candida albicans JLC31680, Candida albicans JLC31681, Escherichia coli ATCC 8099, Staphylococcus aureus ATCC 6538 have different degrees of inhibition. Among them, the water extract had the strongest inhibitory effect on Staphylococcus aureus ATCC 6538 compared with the other five extracts, followed by E. coli ATCC 8099 at the concentration of 100 mg.mL-1, the inhibitory rates were 95.54% And 73.80% respectively, the minimum inhibitory concentration (MIC) was 50 mg.mL-1 and 25 mg.mL-1, respectively. The inhibitory effects on Candida albicans JLC31680 and Candida albicans JLC31681 were also obvious. When the concentration was 100 mg.mL-1, the inhibitory rates were 33.06% and 26.67%, respectively, and the minimum inhibitory concentrations (MICs) were 12.5 mg .mL-1, 50 mg.mL-1. Methanol extract had the strongest inhibitory effect on Escherichia coli ATCC 8099 and Staphylococcus aureus ATCC 6538 compared with the other five extracts. When the concentration was 50 mg.mL-1, the inhibitory rates were 99.39% and 95.10, respectively %, The minimum inhibitory concentration (MIC) is 12.5 mg.mL-1, 25 mg.mL-1, and have a dose-effect relationship.