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目的:研究阿魏酸钠(SF)在心肌缺血/再灌注损伤中的保护作用机制。方法:40只SD大鼠随机分为假手术组、模型组和SF组(结扎后5min注射SF),采用左冠状动脉前降支结扎法制备大鼠心肌缺血/再灌注模型,以免疫酶标法检测大鼠血清内缺血/再灌注肌酸激酶同工酶(CK-MB)和诱导型一氧化氮合酶(iNOS)的含量;以TUNEL(FITC)法检测心肌细胞的凋亡指数;并用半定量PCR的方法检测心肌组织内Bcl-2蛋白的表达。结果:与模型组相比,SF能降低心肌缺血/再灌注后CKMB及iNOS的含量(P<0.05),降低缺血部位凋亡心肌细胞的数量(P<0.05),促进Bcl-2蛋白的表达。结论:与其他报道一致,SF处理后,心肌细胞抗氧化能力增强;除此之外,心肌细胞内凋亡调节蛋白Bcl-2的表达增加,缺血局部凋亡细胞数量降低,从而减轻心肌缺血/再灌注部位的损伤,维持正常的心肌功能。本研究提示,心肌缺血/再灌注前使用SF的治疗,将有助于保护缺血心肌和心肌功能的恢复。
Objective: To study the protective effect of sodium ferulate (SF) on myocardial ischemia / reperfusion injury. Methods: Forty SD rats were randomly divided into sham operation group, model group and SF group (SF injected 5 min after ligation). Rat models of myocardial ischemia / reperfusion were prepared by ligation of left anterior descending coronary artery. The contents of CK-MB and iNOS in the serum were detected by the standard method. The apoptotic index of cardiomyocytes was detected by TUNEL (FITC) . The expression of Bcl-2 protein in myocardium was detected by semi-quantitative PCR. Results: Compared with the model group, SF decreased the levels of CKMB and iNOS (P <0.05), decreased the number of apoptotic cardiomyocytes (P <0.05), and promoted the expression of Bcl-2 protein expression. CONCLUSIONS: Consistent with other reports, the anti-oxidative capacity of cardiomyocytes increased after SF treatment. In addition, the expression of Bcl-2, an inhibitor of apoptosis, increased in cardiomyocytes and the number of apoptotic apoptotic cells decreased, thereby reducing myocardial shortcomings Blood / reperfusion injury site to maintain normal myocardial function. This study suggests that the use of SF prior to myocardial ischemia / reperfusion will help to protect ischemic myocardium and myocardial function recovery.