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为了揭示东乡野生稻芒长的遗传规律。本研究以“东乡野生稻/93-11”F_1、F_2和F_(2:3)群体为遗传材料,构建了1张含163个SSR标记的遗传连锁图谱。考查2群体各株系芒长性状值,利用SPSS 19.0、Mapmaker/Exp 3.0和WinQTLCart 2.50软件进行数据统计分析、遗传图谱绘制和QTL定位。结果表明,有芒对无芒在F_1植株呈显性遗传,稻芒的长短在F_2和F_(2:3)群体中呈连续正态分布,表现为数量性状遗传。在F_2和F_(2:3)群体中共检测到7个芒长QTLs,分布在第3、第4、第5、第8、第10和第11号染色体上,贡献率介于1.93%~56.68%。其中,第4号染色体RM5687标记附近两群体均检测到1个芒长QTL(qAL4a和qAL4b)。在F_2和F_(2:3)群体中共检测到3对芒长上位性QTLs。芒长以加/显/上位性效应遗传模式共同作用,遗传基础较复杂。本研究所检测到的QTL为揭示东乡野生稻芒长进化的遗传机理奠定基础。
In order to reveal the genetic law of wild rice in Dongxiang. In this study, a genetic linkage map with 163 SSR markers was constructed based on the F_1, F_2 and F_ (2: 3) populations from the “Dongxiang Wild Rice / 93-11”. The Mongolian mandarin characters were tested in two populations. Statistical analysis, genetic mapping and QTL mapping were performed using SPSS 19.0, Mapmaker / Exp 3.0 and WinQTLCart 2.50 software. The results showed that M. mandshurica had a dominant inheritance in F_1 plants. The length of O. mandarin was continuously normal distribution in F_2 and F_ (2: 3) populations, showing the inheritance of quantitative traits. Seven M. longum QTLs were detected in F_2 and F_ (2: 3) populations on chromosomes 3, 4, 5, 8, 10 and 11 with a contribution rate ranging from 1.93% to 56.68 %. Among them, one M. longum QTL (qAL4a and qAL4b) was detected in two populations near RM5687 marker on chromosome 4. Three pairs of mtDNA epistatic QTLs were detected in F_2 and F_ (2: 3) populations. Mansions to plus / significant / epistatic effects of genetic models work together, the genetic basis is more complicated. The QTLs detected in this study laid the foundation for revealing the genetic mechanism of the Mongolian wilderness evolution in Dongxiang.