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目的测定中药复方制剂灯盏生脉提取物灌胃后大鼠血浆中野黄芩苷的浓度。方法采用液-液萃取法制备血液样品。以芦丁为内标,采用C18柱,乙腈-体积分数为0.05%的磷酸(体积比为65∶320)为流动相,流速为1.0 mL.min-1,检测波长为335 nm,柱温为40℃。结果野黄芩苷血浆中质量浓度在0.05~5.00 mg.L-1(r=0.997 9),定量下限为0.05 mg.L-1,日内、日间精密度均小于15%。在质量浓度为0.1、0.5、4.0 mg.L-1时,提取回收率分别为(81.6±3.6)%、(79.2±3.0)%和(78.7±2.4)%。主要药动学参数为:ke=(0.269±0.035)h-1、t1/2=(2.61±0.36)h、AUC0-24=(25.272±5.682)mg.h.L-1。结论该方法可用于灯盏生脉提取物中野黄芩苷的药动学研究。
Objective To determine the concentration of baicalin in the plasma of rats after oral administration of the Chinese traditional medicine compound Dengzheng Shengmai extract. Methods Blood samples were prepared by liquid-liquid extraction. Rutin was used as internal standard. The mobile phase consisted of C18 column, acetonitrile-0.05% phosphoric acid (volume ratio of 65:320), the flow rate was 1.0 mL.min-1, the detection wavelength was 335 nm, 40 ° C. Results The concentration of baicalin in plasma was 0.05-5.50 mg.L-1 (r = 0.997 9), and the lower limit of quantitation was 0.05 mg.L-1. The intra-day and inter-day precision were less than 15%. The recoveries were (81.6 ± 3.6)%, (79.2 ± 3.0)% and (78.7 ± 2.4)%, respectively, at the concentrations of 0.1, 0.5 and 4.0 mg.L-1. The main pharmacokinetic parameters were ke = (0.269 ± 0.035) h-1, t1 / 2 = (2.61 ± 0.36) h and AUC0-24 = (25.272 ± 5.682) mg.h.L-1. Conclusion This method can be used for the pharmacokinetics of scutellarin in Dengzhan Shengmai extract.