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目的建立测定旱芹非淀粉多糖中各单糖组分含量的柱前衍生化GC法。方法非淀粉多糖经衍生化生成糖腈乙酯,采用GC法进行分析。色谱柱:DB-17柱(30 m×0.25 mm,0.25μm);柱温:程序升温;载气:N2;进样温度:270℃;进样方式:不分流;检测器温度:270℃;进样量:1.0μL。结果水不溶性非淀粉多糖(insoluble non-starch polysaccharides,INSP)水解后得到7种单糖,分别是:L-(+)-鼠李糖、L-(+)-阿拉伯糖、D-(+)-岩藻糖、D-(+)-木糖、D-(+)-甘露糖、D-(+)-葡萄糖和D-(+)-半乳糖;水溶性非淀粉多糖(soluble non-starch polysaccharides,SNSP)水解后得到6种单糖,除无D-(+)-岩藻糖外,其余均同INSP水解后单糖。结论本方法准确度高,重现性好,可用于测定旱芹水溶性和水不溶性非淀粉多糖中各单糖组分的含量。
OBJECTIVE To establish a pre-column derivatization GC method for determining the content of monosaccharides in non-starch polysaccharides of Celery. Methods The non-starch polysaccharides were derivatized to give the ethyl ester of saccharin and analyzed by GC method. Column: DB-17 column (30 m × 0.25 mm, 0.25 μm); Column temperature: programmed temperature; Carrier gas: N2; Injection temperature: 270 ℃; Injection mode: Splitless; Detector temperature: 270 ℃; Injection volume: 1.0μL. Results Seven kinds of monosaccharides were obtained after hydrolysis of insoluble non - starch polysaccharides (INSP), which were L - (+) - rhamnose, L - D - (+) - mannose, D - (+) - glucose and D - (+) - galactose; soluble non-starch Polysaccharides and SNSP were hydrolyzed to obtain six kinds of monosaccharides, except for D - (+) - fucose. Conclusion The method has high accuracy and reproducibility and can be used to determine the content of each monosaccharide in water-soluble and water-insoluble non-starch polysaccharides.