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本文研究不同浓度（１０－１０—１０－４ｍｏｌ／Ｌ）乙酰胆碱（ＡＣｈ）对离体培养的大鼠脾脏Ｔ淋巴细胞增殖的影响，并探讨其作用机制。实验结果表明：１０－９—１０－４ｍｏｌ／ＬＡＣｈ可显著增强Ｔ细胞由刀豆素Ａ（ＣｏｎＡ）诱导的增殖反应，以１０－７—１０－６ｍｏｌ／Ｌ时最强。淋巴细胞先用ＡＣｈ刺激１ｈ或者刺激１ｈ后洗弃ＡＣｈ，再用ＣｏｎＡ诱导７２ｈ，增殖反应亦加强，但ＡＣｈ不能加强被ＣｏｎＡ诱导６ｈ的Ｔ细胞的增殖。１０－７—１０－６ｍｏｌ／Ｌ阿托品可完全阻断１０－９ｍｏｌ／Ｌ或１０－５ｍｏｌ／ＬＡＣｈ的免疫增强效应，１０－６ｍｏｌ／Ｌ阿托品可阻断１０－７ｍｏｌ／ＬＡＣｈ的作用。上述结果提示，ＡＣｈ在较广的浓度范围内均可促进细胞免疫功能，这种调节作用发生于Ｔ细胞激活前或激活的早期，是通过Ｍ型胆碱能受体实现的。
The aim of this study was to investigate the effects of different concentrations (10-10-10-4 mol / L) of acetylcholine (ACh) on the proliferation of splenic T lymphocytes in vitro and its possible mechanism. The experimental results showed that 10-9-10-4 mol / LACh could significantly enhance the proliferation of T cells induced by ConA, and the strongest was 10-7-10-6 mol / L. Lymphocytes were stimulated with ACh for 1h or ACh for 1h, and then stimulated with ConA for 72h. The proliferative response was also enhanced. However, ACh did not enhance the proliferation of T cells induced by ConA for 6h. 10-7-10-6mol / L atropine can completely block 10-9mol / L or 10-5mol / LACh immune enhancement effect, 10-6mol / L atropine can block the role of 10-7mol / LACh. The above results suggest that ACh can promote cellular immune function in a wide range of concentrations, which occurs before or during T cell activation through the M cholinergic receptor.