论文部分内容阅读
报道了一种灵敏的氧氟沙星测定方法──一阶导数单扫示波极谱分析，同时研究了它的极谱波性质和电极反应机理。在ｐＨ ６．０的ＫＨ２ＰＯ４－Ｎａ２ＨＰＯ４（ＰＢＳ）底液中，氧氟沙星能产生一个灵敏的还原峰，Ｅｐ＝－１．５５ Ｖ（ｖｓ．ＳＣＥ），其导数峰高与浓度在１．０ × １０－５～４．６×１０－７ｍｏｌ／Ｌ范围内呈良好的线性关系，相关系数ｒ＝０．９９９６，检测下限为 ２．４×１０－７ｍｏｌ／Ｌ，可用于氧氟沙星片剂的测定，ＲＳＤ％＝０．４，回收率为９７．３％～１０４．９％。此法还用于血清及尿样中痕量氧氟沙星的测定，结果都与紫外法基本吻合。经多种实验证明，其电极过程为反应物吸附的不可逆过程，电子转移数为２，电子转移系数为０．５３，参与电极反应的Ｈ＋数为１，此外还研究了人体内共存微量物质对ｉ＇ｐ的影响。
A sensitive method for ofloxacin, a first derivative single sweep sweep polarographic analysis, was reported, and its polarographic wave properties and electrode reaction mechanism were also studied. Ofloxacin produced a sensitive reduction peak at pH 6.0 in KH2PO4-Na2HPO4 (PBS), Ep = -1.55 V (vs.SCE) with a derivative peak height of 1. 0 × 10-5 ~ 4.6 × 10-7mol / L showed a good linear relationship, the correlation coefficient r = 0.9996, the detection limit of 2.4 × 10-7mol / L, can be used for ofloxacin The determination of tablets, RSD% = 0.4, recovery was 97.3% ~ 104.9%. This method is also used for determination of ofloxacin in serum and urine samples, the results are basically consistent with the UV method. It has been proved by a variety of experiments that the electrode process is an irreversible process of reactant adsorption. The number of electron transfer is 2, the electron transfer coefficient is 0.53, the number of H + participating in the electrode reaction is 1. In addition, i’p influence.