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目的通过对食源性致病菌质量控制的结果分析,总结出食源性致病菌分离鉴定的经验,提高食源性致病菌分离鉴定的能力。方法按照常见食源性致病菌分离鉴定的方法进行分离鉴定。结果真空冻干保存的菌种经前增菌培养比未经前增菌培养在液体培养基中生长更快,在分离鉴定培养基上易形成典型菌落特征,更便于食源性致病菌分离鉴定;用常见的肠道致病菌分离鉴定培养基可分离鉴定出肠出血性大肠埃希菌O157∶H7。结论真空冻干保存的菌种分离培养前,先进行5~6 h前增菌培养,有利于细菌的复苏和特征菌落的形成;几种常见的肠道致病菌分离鉴定培养基,本来对大肠埃希菌有抑制作用,但肠出血性大肠埃希菌O157∶H7却生长良好,反而有利于肠出血性大肠埃希菌O157∶H7的分离鉴定,为基层实验室分离肠出血性大肠埃希菌提供了一种便利的方法。
Objective To analyze the results of quality control of food-borne pathogens, summarize the experience of isolation and identification of food-borne pathogens and improve the ability of isolation and identification of food-borne pathogens. Methods Isolation and identification of common food-borne pathogens were carried out. Results The results showed that the growth of the vacuum freeze-dried strain was faster than that of the non-pre-enrichment culture in liquid medium, and typical colony characteristics were easily formed on the medium for isolation and identification, which was more convenient for the isolation of food-borne pathogens Identification; Enteromycete Escherichia coli O157: H7 can be isolated and identified with the common intestinal pathogenic bacteria isolation and identification medium. CONCLUSIONS: Bacteria cultured in vacuum freeze-dried before isolation and culture are cultured for 5 ~ 6 h prior to enrichment, which is beneficial to the recovery of bacteria and the formation of characteristic colonies. Several common intestinal pathogenic bacteria isolation and identification medium, originally Escherichia coli has inhibitory effect, but enterohemorrhagic Escherichia coli O157: H7 has grown well, but conducive to the separation and identification of enterohemorrhagic Escherichia coli O157: H7 separation of primary hemorrhagic colon for laboratory Xymph provides a convenient way.