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目的:探讨结核杆菌PhoP R双组分系统与新疆耐药结核杆菌耐药性的相关性。方法:新疆耐异烟肼、耐利福平、耐链霉素、耐乙胺丁醇、耐多药结核杆菌及药物敏感菌株分别行RT-PCR,测原始状态及抗痨药物选择压力下PhoP和PhoR基因表达及差异;复制各菌株动物模型并从肺、肝、脾分离菌株,测PhoP和PhoR基因表达及差异。结果:耐药结核杆菌PhoP和PhoR
Objective: To investigate the relationship between Mycobacterium tuberculosis PhoP R two-component system and drug-resistant Mycobacterium tuberculosis in Xinjiang. Methods: The resistance to isoniazid, rifampicin, streptomycin, ethambutol, multidrug-resistant Mycobacterium tuberculosis and drug-susceptible strains were determined by RT-PCR in Xinjiang and the PhoP And PhoR gene expression and differences. The animal models of each strain were replicated and the isolates were isolated from lung, liver and spleen. The expression and difference of PhoP and PhoR genes were examined. Results: M. tuberculosis PhoP and PhoR