用ZEN-BSA人工抗原免疫BALB/c鼠,经融合、筛选和克隆化得到可稳定分泌抗ZEN单克隆抗体的杂交瘤细胞株ZEN-1C6。ZEN-1C6属IgG_1,纯化腹水抗体效价为10~(-5),与5种衍生物的交叉反应系数为0.16～1.20％。用ZEN-1C6建立了检测食品(玉米、小麦、大米)中玉米赤霉烯酮的CIEIA法。该法检测纯毒素的线性范围为5～1000ng/ml,最低检出浓度为0.1ng/ml,平均回收率为84.0～105.5％。用该法测定了81份样品,均有ZEN毒素检出。 BALB / c mice were immunized with ZEN-BSA artificial antigen and fused, screened and cloned to obtain a hybridoma cell line ZEN-1C6 capable of stably secreting anti-ZEN monoclonal antibody. ZEN-1C6 belongs to IgG_1, the purified ascites antibody titer is 10 ~ (-5), and the cross-reaction coefficient with the five derivatives is 0.16 ~ 1.20%. The CIEIA method for the detection of zearalenone in foodstuffs (corn, wheat, rice) was established with ZEN-1C6. The linear range of this method was 5 ~ 1000ng / ml, the lowest detection limit was 0.1ng / ml, and the average recovery was 84.0 ~ 105.5%. With this method, 81 samples were determined, all of which were detected by ZEN toxin.