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目的探讨热休克蛋白（ｈｅａｔｓｈｏｃｋｐｒｏｔｅｉｎｓ，ＨＳＰｓ）在喉癌组织及其正常喉粘膜中的表达。方法采用狭缝杂交（Ｓｌｏｔｂｌｏｔａｎａｌｙｓｉｓ）技术对２１例喉癌组织及其正常喉粘膜的ＨＳＰ９０、ＨＳＰ７０和ＨＳＰ２７ｍＲＮＡ表达水平进行检测。结果①ＨＳＰ９０α和ＨＳＰ７０在喉癌组织中呈过度表达，表达量是自身正常喉粘膜的５倍以上。②ＨＳＰ２７和ＨＳＰ９０β在喉癌组织及正常喉粘膜中呈低水平表达，其表达量在两种组织中差异无显著性。结论ＨＳＰ７０和ＨＳＰ９０α在喉癌的致病过程中起着重要的作用。弄清它们的作用机理，将为ＨＳＰｓ用于喉癌的生物治疗提供理论依据。
Objective To investigate the expression of heat shock proteins (HSPs) in laryngeal carcinoma and its normal laryngeal mucosa. Methods The expression of HSP90, HSP70 and HSP27 mRNA in 21 cases of laryngeal carcinoma and its normal laryngeal mucosa were detected by Slotblotanalysis. Results ① HSP90α and HSP70 were overexpressed in laryngeal squamous cell carcinoma and the expression was over 5 times higher than that of normal laryngeal mucosa. ② The expression of HSP27 and HSP90β in laryngeal carcinoma and normal laryngeal mucosa was low, and the expression level was not significantly different between the two tissues. Conclusion HSP70 and HSP90α play an important role in the pathogenesis of laryngeal cancer. To clarify their mechanism of action, will provide a theoretical basis for the biological treatment of HSPs for laryngeal cancer.