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阿魏酸在溶剂中不稳定,这给定量分析带来一定干扰.研究了阿魏酸在6种不同溶剂系统中的稳定性,改进了溶解阿魏酸的溶剂,在此基础上建立了生药当归中阿魏酸的高效毛细管区带电泳分离分析方法.电泳条件:以60mmol/L硼砂(pH9.45)为电泳介质,未涂层弹性融硅毛细管(50μm×39.5cm,有效分离长度34.8cm)为分离通道,压力进样(68.95kPa·s),20kV恒压电泳(25℃),检测波长314um.实验发现,在4mg/L～400mg/L范围内,阿魏酸可进行定量分析,加样回收率为100.4±1.2%.方法简便快速、结果准确,重现性好,可用于当归及其制剂的质量控制.
Ferulic acid is unstable in the solvent, which brings some interference to the quantitative analysis.We studied the stability of ferulic acid in six different solvent systems, improved the solvent of ferulic acid dissolved, on this basis, established the crude drug High performance capillary electrophoresis separation and analysis of ferulic acid from Angelica sinensis Angelica sinensis.Electrophoretic conditions: using 60mmol / L borax pH9.45 as electrophoresis medium, uncoated flexible fused silica capillary (50μm × 39.5cm, effective separation length of 34.8cm ) For the separation channel, the pressure of the sample (68.95kPa · s), 20kV constant voltage electrophoresis (25 ℃), the detection wavelength of 314um.Experiment found that in the range of 4mg / L ~ 400mg / L, ferulic acid can be quantitative analysis, The recovery rate was 100.4 ± 1.2%. The method is simple, rapid, accurate and reproducible. It can be used for the quality control of Angelica sinensis and its preparations.