论文部分内容阅读
用改进的差异显示反转录-PCR技术，从血管内皮细胞中获得了一个氧化低密度脂蛋白诱导差异表达的cDNA 片段。以此片段为探针，从人主动脉cDNA 文库中筛选了一个新的全长cDNA 。其核苷酸总长4137bp， 编码642 个氨基酸的蛋白质。Northern 印迹分析证实氧化低密度脂蛋白诱导内皮细胞后，该基因表达减低了3 倍，基因定名为OLRG-1。OLRG-1 为一多组织表达的基因，与人NS1-BP 基因高度同源。氧化低密度脂蛋白可能通过降低OLRG-1 的表达，影响mRNA 前体的剪接成熟，调控基因的表达。
A modified differential display reverse transcription-PCR technique was used to obtain a differentially expressed cDNA fragment induced by oxidized low density lipoprotein from vascular endothelial cells. Using this fragment as a probe, a new full-length cDNA was screened from a human aortic cDNA library. The nucleotide length of 4137bp, encoding a protein of 642 amino acids. Northern blot analysis confirmed that the gene expression was reduced by a factor of 3 when oxidized low-density lipoprotein induced endothelial cells and the gene was designated OLRG-1. OLRG-1 is a multi-tissue expressed gene that is highly homologous to the human NS1-BP gene. Oxidized low-density lipoprotein may regulate gene expression by decreasing the expression of OLRG-1, affecting the splicing maturation of mRNA precursor.