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本文提出了一种新的评价色谱指纹图谱分离质量的指标,即改进的校正标准分离度积(ri*’)。以大豆异黄酮提取物为分析对象,通过正交设计对高效液相梯度洗脱的初始甲醇比例、洗脱结束时的甲醇比例以及洗脱时间进行考察,用改进的校正标准分离度积(ri*’)衡量所得色谱图,通过与校正标准分离度积(ri*)及谱系色谱响应函数(HCRF)进行比较,分析各个指标的优缺点,论证了改进的校正标准分离度积(ri*’)这一评估指标的有效性,并建立了大豆异黄酮提取物指纹图谱的优化色谱条件(流动相A相:0.1%醋酸,B相:甲醇;梯度洗脱B相:;流速:1.0 m L/min检测波长:254 nm;柱温:36 oC)。此外,用高效液相色谱-电喷雾质谱法对大豆异黄酮提取物的活性成分进行了归属分析。25 min34%????65%
In this paper, we propose a new index to evaluate the quality of chromatographic fingerprinting separation, namely, the improved calibration standard resolution product (ri * ’). Taking the soy isoflavone extract as the analysis object, the orthogonal design was used to study the ratio of initial methanol, the proportion of methanol at the end of elution, and the elution time. The modified calibration standard * ’) Was used to measure the obtained chromatogram. The advantages and disadvantages of each index were analyzed by comparison with calibration standard resolution product (R *) and spectral response function (HCRF). The improved calibration standard resolution product (ri * ), And established the optimized chromatographic conditions of the fingerprint of soy isoflavone extract (mobile phase A: 0.1% acetic acid, phase B: methanol; gradient elution phase B: flow rate: 1.0 mL / min detection wavelength: 254 nm; column temperature: 36 oC). In addition, the active constituents of the soy isoflavone extract were analyzed by high performance liquid chromatography-electrospray ionization mass spectrometry. 25 min34% ???? 65%