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目的探讨缺氧诱导因子-1α(HIF-1α)与基因系尾型同源盒基因2(CDX2)蛋白在结直肠腺癌组织中的表达及其与结直肠癌发生、发展的关系,并且研究HIF-1α与CDX2表达之间的相关性。方法收集62例结直肠腺癌及20例正常结直肠组织标本,采用免疫组化SABC法检测标本中HIF-1α、CDX2蛋白的表达,并采用Spearman等级相关分析分析HIF-1α与CDX2表达之间的相关性。结果在20例正常结直肠黏膜组织中HIF-1α及CDX2蛋白表达阳性率分别为5.0%(1/20)及95.0%(19/20),在62例结直肠腺癌组织中HIF-1α及CDX2蛋白表达阳性率分别为62.9%(39/62)及69.4%(43/62),不同组织中蛋白表达的差异均有统计学意义(P<0.05)。结直肠腺癌中,HIF-1α、CDX2蛋白的表达与肿瘤的分化程度、淋巴结转移及Dukes分期有关(P<0.05);Spearman等级相关分析表明,HIF-1α与CDX2的表达呈负相关(r=-0.293 2,P<0.05)。结论 HIF-1α表达上调、CDX2表达下调可能与结直肠腺癌的发生有关,且这两者间呈负相关。HIF-1α可能参与调控CDX2的表达,进而加速了结直肠癌的恶性进展。
Objective To investigate the expression of hypoxia inducible factor-1α (HIF-1α) gene and tail-tailed homologous box 2 (CDX2) gene in colorectal adenocarcinoma and its relationship with the occurrence and development of colorectal cancer. Correlation between HIF-1α and CDX2 expression. Methods Totally 62 cases of colorectal adenocarcinoma and 20 cases of normal colorectal tissues were collected. The expression of HIF-1α and CDX2 protein was detected by immunohistochemical SABC method. The expression of HIF-1α and CDX2 was analyzed by Spearman rank correlation analysis Relevance. Results The positive rates of HIF-1α and CDX2 in 20 cases of normal colorectal mucosa were 5.0% (1/20) and 95.0% (19/20), respectively. In 62 cases of colorectal adenocarcinoma, the positive rates of HIF-1α and The positive rates of CDX2 protein were 62.9% (39/62) and 69.4% (43/62), respectively. There were significant differences in the expression of CDX2 between different tissues (P <0.05). The expression of HIF-1αand CDX2 in colorectal adenocarcinoma correlated with tumor differentiation, lymph node metastasis and Dukes stage (P <0.05). Spearman rank correlation analysis showed that the expression of HIF-1α and CDX2 were negatively correlated = -0.293 2, P <0.05). Conclusion The up-regulation of HIF-1α expression and the down-regulation of CDX2 may be related to the occurrence of colorectal adenocarcinoma, and there is a negative correlation between them. HIF-1α may be involved in the regulation of CDX2 expression, thereby accelerating the malignant progression of colorectal cancer.