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目的 :建立一种便捷的检测系统性红斑狼疮 (SLE)患者血清抗双链DNA抗体的方法。方法 :在斑点免疫金银染色法 (dot-immungold -silverstaining ,Dot-IGSS)的基础上 ,缩短反应和洗涤时间 ,建立微量快速斑点免疫金银染色法 (Microvolume -Fast -Dot-IGSS ,MFDot-IGSS)并与Dot-IGSS进行比较。结果 :用MF Dot-IGSS和Dot-IGSS同步检测 70份SLE患者血清抗双链DNA抗体 (A -dsDNA) ,阳性率分别 6 4 .3%和6 5 .7% ;检测其他自身免疫性疾病患者血清 30份阳性率均为 3.3% ;检测健康献血员血清 5 0份 ,阳性率均为2 .0 %。二种方法的敏感性和特异性均无显著性差异 (P >0 .0 5 )。结论 :MFDot -IGSS检测SLE病人血清A-dsDNA的敏感性和特异性与Dot -IGSS相似 ,但便简快速 ,适用于门诊及基层医院。
Objective: To establish a convenient method of detecting serum anti-double-stranded DNA antibody in patients with systemic lupus erythematosus (SLE). Methods: On the basis of Dot-immung-silverstaining (Dot-IGSS), the reaction and washing time were shortened and the microvolume-Fast-Dot-IGSS IGSS) and compared with Dot-IGSS. Results: Seventy-two patients with SLE were detected simultaneously by MF Dot-IGSS and Dot-IGSS. The positive rates of A-dsDNA were 64.3% and 65.7% respectively. The detection of other autoimmune diseases The positive rate of 30 patients was 3.3%, and the serum of healthy donors was 50, the positive rate was 2.0%. The sensitivity and specificity of the two methods had no significant difference (P> 0.05). CONCLUSION: The sensitivity and specificity of MFDot-IGSS for the detection of serum A-dsDNA in SLE patients are similar to that of Dot-IPS. However, they are simple and rapid and are suitable for outpatients and primary hospitals.