设计合成了1-(2-羟基苯基)-4-(9-蒽基)-3-苯并噻唑基吡唑啉(P1),通过紫外吸收和荧光光谱法研究了化合物与金属离子的识别特性,并研究了其在PK-15活细胞中进行荧光显微成像实验.研究结果表明荧光探针P1能快速、高选择、可逆地识别Cu~(2+),细胞毒性实验和荧光免疫分析实验证明,所制备的荧光探针具有很好的生物相容性和低毒性. Design and synthesis of 1- (2-hydroxyphenyl) -4- (9-anthracenyl) -3-benzothiazolyl pyrazoline (P1). The compounds and metal ions were identified by UV and fluorescence spectroscopy And its fluorescence microscopy in PK-15 living cells was studied.The results showed that the fluorescent probe P1 can rapidly, selectively and reversibly recognize Cu 2+, cytotoxicity and fluorescence immunoassay Experiments show that the prepared fluorescent probe has good biocompatibility and low toxicity.