在肝炎病毒分离研究过程中,我们试用一些东北地区特产动物的脏器做组织培养,如用貂、狐、梅花鹿等动物的肾、睾丸、心肌组织,或是单层,或是组织块培养,尚未得到满意结果。当时我们也考虑到特产动物的血清,对其他常用的组织细胞培养是否有效。1961年夏季,在吉林省龙潭山鹿场,调查鹿的几种疾病时期,遇到茸鹿(梅花鹿)锯茸的机会,得到了多量的鹿血清,经滤过灭活处理后,用来代替牛血清作组织培养,首先试用于鸡胚单层细胞,感觉到细胞生长良好,不次于牛血清,后来又试用于人肾单 In the process of isolation and research of hepatitis virus, we try to organize the tissue culture of some animals in the northeast region, such as the kidney, testis, myocardial tissue, or monolayer, or tissue block culture of animals such as quail, fox and spotted deer. Not yet satisfied with the results. At the time, we also considered whether the serum of special animals was effective for other commonly used tissue cell cultures. In the summer of 1961, in the deer field of Longtan Mountain in Jilin Province, the deer disease was investigated and the chance of sawing antler was encountered. A large amount of deer serum was obtained and used as a substitute for cattle after filtration and inactivation. The serum was used for tissue culture. It was first tested on chick embryo monolayer cells, and the cell growth was felt to be good, not inferior to bovine serum. Later it was tested on human kidneys.