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光功率密度的大小直接决定耗氧量的多少,在光动力疗法(PDT)中的作用至关重要。为了研究光功率密度对PDT制备肿瘤疫苗效果的影响,研究采用两种功率密度(5mW/cm2和25mW/cm2)的激光,能量密度分别为2J/cm2和10J/cm2。实验表明:在5mW/cm2×2J/cm2条件下制备的疫苗抑瘤率可以达到70%～80%,而25mW/cm2×2J/cm2制备的疫苗抑瘤率只有40%～50%;5mW/cm2×10J/cm2制备的肿瘤疫苗抑瘤率为25%～30%,25mW/cm2×10J/cm2制备的疫苗抑瘤率为20%～25%。结果表明:与高功率密度激光相比,低功率密度制备的肿瘤疫苗更能有效刺激小鼠的免疫系统,使得CD4+、CD8+、CD19+的细胞数增加。在功率密度为5mW时,能量密度达到2J/cm2,制备的肿瘤疫苗能够较大程度地刺激小鼠的免疫系统,对肿瘤的抑制最为有效。
The size of the optical power density directly determines the amount of oxygen consumption, the role of photodynamic therapy (PDT) is crucial. To study the effect of optical power density on the efficacy of PDT in the preparation of tumor vaccines, two power densities (5mW / cm2 and 25mW / cm2) were studied with energy densities of 2 J / cm2 and 10 J / cm2, respectively. The results showed that the tumor inhibition rate could reach 70% ~ 80% under the condition of 5mW / cm2 × 2J / cm2, while that of the vaccine prepared by 25mW / cm2 × 2J / cm2 was only 40% ~ 50% The inhibition rate of the tumor vaccine prepared by the tumor vaccine is 25% -30%, and the tumor inhibition rate of the vaccine prepared by 25mW / cm2 × 10J / cm2 is 20% -25%. The results show that the tumor vaccine prepared by low power density can effectively stimulate the immune system of mice compared with high power density laser, and increase the number of CD4 +, CD8 + and CD19 + cells. At a power density of 5mW, the energy density reached 2J / cm2. The prepared tumor vaccine can stimulate the immune system to a greater degree and inhibit the tumor most effectively.