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背景与目的:已证实阿霉素热化疗对体外兔VX-2细胞的抑制作用比常温阿霉素的作用强,而热灌注对机体的生命体征有一定影响。本研究在兔VX-2移植瘤模型建立基础上,比较间歇性热灌注与连续性热灌注对兔呼吸、心率、体温及VX-2肿瘤内阿霉素浓度的影响,验证间歇性热灌注的有效性和安全性,以寻找更安全有效的灌注方式。方法:在30只新西兰大白兔后腿上建立VX-2肿瘤模型,并随机分为常温灌注组、60℃连续灌注组和60℃间歇灌注组(每组10只)。经股动脉插管、DSA证实为肿瘤供血动脉后,分别给予常温100ml盐水加阿霉素(ADM)灌注、60℃热盐水100ml加阿霉素连续灌注、60℃热盐水100ml加阿霉素间歇性灌注。灌注过程中,测量60℃热灌注组与60℃间歇灌注组肿瘤组织内43℃～45℃持续时间;灌注后即时检测各组兔呼吸(次/分)、心率(次/分)、体温(℃)及肿瘤组织内阿霉素浓度。结果:常温灌注组阿霉素浓度为(7.115±2.180)μg/ml,60℃连续灌注组为(17.213±1.657)μg/ml,60℃间歇性灌注组为(16.545±3.426)μg/ml;60℃间歇灌注组阿霉素浓度与60℃连续灌注组无显著性差异(P>0.05),60℃连续组、间歇组阿霉素浓度与常温灌注组均有显著性差异(P<0.05)。60℃间歇性灌注组43℃～45℃持续时间为(24.31±2.45)min,60℃连续灌注组为(22.53±1.
BACKGROUND & OBJECTIVE: It has been confirmed that adriamycin thermo-chemotherapy can inhibit the VX-2 cells in vitro more effectively than do adriamycin at normal temperature, and that hot perfusion has some effect on the vital signs of the body. In this study, based on the establishment of the rabbit VX-2 xenograft model, we compared the effects of intermittent and continuous thermal perfusion on respiration, heart rate, body temperature and doxorubicin concentration in VX-2 tumors. Effectiveness and safety in order to find more safe and effective perfusion method. Methods: VX-2 tumor models were established on the hind legs of 30 New Zealand white rabbits and were randomly divided into normal temperature perfusion group, 60 ℃ continuous perfusion group and 60 ℃ intermittent perfusion group (10 rats in each group). After femoral artery cannulation, DSA confirmed that the tumor feeding artery, were given normal saline 100ml saline plus doxorubicin (ADM) perfusion, 60 ℃ hot saline 100ml continuous infusion of doxorubicin, 60 ℃ hot saline 100ml with doxorubicin intermittent Perfusion. During perfusion, the duration of 43 ° C ~ 45 ° C in the tumor tissue at 60 ° C and intermittent perfusion group at 60 ° C was measured. The levels of respiration (subscales), heart rate (subscales), body temperature ℃) and doxorubicin concentration in tumor tissue. Results: The concentrations of doxorubicin in normal temperature group were (7.115 ± 2.180) μg / ml and those in continuous group at 60 ℃ were (17.213 ± 1.657) μg / ml and those in intermittent group at 60 ℃ were (16.545 ± 3.426) μg / ml, The concentration of doxorubicin in intermittent perfusion group at 60 ℃ was not significantly different from that in continuous perfusion group at 60 ℃ (P> 0.05), but there was a significant difference between doxorubicin group and intermittent perfusion group at 60 ℃ (P <0.05) . The duration of 43 ℃ ~ 45 ℃ in intermittent perfusion group at 60 ℃ was (24.31 ± 2.45) min, and the continuous perfusion group at 60 ℃ was (22.53 ± 1.