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应用雄性Wistar大鼠腹腔注射链脲霉素诱导DN动物模型,造模成功后分为模型组、鹿茸方小剂量治疗组、鹿茸方中剂量治疗组、水飞蓟宾治疗组及正常对照组。干预治疗8周后,测定各组大鼠24小时尿微量白蛋白排泄量(UAER);用RT-PCR法测定各组大鼠RGN mRNA的表达。结果:模型组大鼠UAER显著增高、肾皮质RGN mRNA表达显著降低(P<0.05);②治疗组显著降低模型UAER,显著上调肾皮质RGN mRNA的表达(P<0.05);结论:鹿茸方及对照药物水飞蓟宾均对DN有一定治疗作用,其作用机制与上调肾皮质RGN mRNA的表达有关。
Male Wistar rats were injected intraperitoneally with streptozotocin to induce DN animal model. After successful modeling, they were divided into model group, low-dose antler group, antler group middle-dose group, silybin group and normal control group. After intervention for 8 weeks, the 24-hour urinary albumin excretion (UAER) of rats in each group was measured. The expression of RGN mRNA in each group was determined by RT-PCR. Results: The UAER in model group was significantly increased and the expression of RGN mRNA in renal cortex was significantly decreased (P <0.05). ② The treatment group significantly reduced the expression of UAER and the expression of RGN mRNA in renal cortex (P <0.05). Conclusion: The control drug silybin has a certain role in the treatment of DN, its mechanism of action and upregulation of renal cortex RGN mRNA expression.