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目的了解吉林省2012~2013年诺如病毒(Norovirus,NV)流行型别及感染状况。方法收集长春市儿童医院2012~2013年<5岁住院腹泻患儿的粪便标本,采用逆转录-聚合酶链反应(Reverse Transcription-Polymerase Chain Reaction,RT-PCR)检测粪便标本中的NV,阳性标本进行核苷酸序列测定,构建系统进化树进行分析。结果检测粪便标本共770份,NV阳性139份,总阳性率为18.1%,其中2012年总阳性率为18.6%(72/387),2013年总阳性率为17.5%(67/383),<2岁婴幼儿感染占总标本数的97.1%,全部为基因Ⅱ组(GenogroupⅡ,GⅡ)。PCR产物进行序列测定,取得测序结果的共97份,其中GⅡ.4型53份,占54.6%;GⅡ.3型41份,占42.3%;GⅡ.13型2份,占2.1%;GⅡ.6型1份,占1.0%。2012年检出GⅡ.4 Sydney(悉尼)2012变异株2份,占2012年阳性总数的4.9%;2013年检出26份,占2013年阳性总数的46.4%。结论吉林省存在多种NV基因型的感染,2013年流行优势株为GⅡ.4 Sydney2012变异株。
Objective To understand the prevalence and infection of Norovirus (NV) in Jilin Province from 2012 to 2013. Methods Stool samples of children aged <5 years with hospital-acquired diarrhea in Changchun Children’s Hospital from 2012 to 2013 were collected. NV and stool specimens of stool samples were detected by reverse transcription-polymerase chain reaction (RT-PCR) The nucleotide sequence was determined and the phylogenetic tree was constructed. Results A total of 770 stool specimens were detected, 139 were positive for NV and the total positive rate was 18.1%. The total positive rate was 18.6% (72/387) in 2012 and 17.5% (67/383) in 2013, Two-year-old infants and young children infected accounted for 97.1% of the total number of specimens, all of the genogroup Ⅱ (Genogroup Ⅱ, G Ⅱ). PCR products were sequenced, and a total of 97 sequencing results were obtained. Among them, 53 were GⅡ.4 type, accounting for 54.6%; G Ⅱ 3 type 41, accounting for 42.3%; GⅡ.13 type 2, accounting for 2.1%; GⅡ. 6 type 1, accounting for 1.0%. In 2012, 2 copies of the GII.4 Sydney (Sydney) 2012 variant were detected, accounting for 4.9% of the total positives in 2012; 26 were detected in 2013, accounting for 46.4% of the total positives in 2013. Conclusion There are many genotypes of NV infection in Jilin Province. In 2013, the predominant strain was GⅡ.4 Sydney2012 variant.