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本实验用妊娠13～15d的小鼠胎肝细胞血浆凝块培养法测定人尿提取物红细胞生成素(EPO)生物活性。实验证明,在相同细胞浓度的培养体系中,红系祖细胞(E-CFU。)产率与EPO浓度(在5～100mu/ml范围内)呈高度正相关。以EPO(7A)为参照标准,用E-CFU。方法测得EPO(850323)活性与缺氧多血小鼠标准的体内分析方法所获得结果一致。提取工艺路线具有较好的重复性。冻干EPO制品溶解后保存于—20℃,6周内活性不变。
In this experiment, the human urinary extract erythropoietin (EPO) bioactivity was measured by the method of plasma clot culture of mouse fetal liver cells from 13 to 15 days of pregnancy. Experiments show that in the same cell culture system, the yield of erythroid progenitor cells (E-CFU) is highly positively correlated with the EPO concentration (in the range of 5 to 100 mu/ml). Using EPO (7A) as a reference standard, E-CFU was used. Methods The measured EPO (850323) activity was consistent with the standard in vivo analysis of hypoxic multiple-mouse mice. The extraction process has good repeatability. Freeze-dried EPO products were stored at -20°C after dissolution, and remained unchanged for 6 weeks.