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目的比较PreS1、HBV-DNA、HBeAg反映病毒复制的临床应用价值。方法对疑有HBV病毒复制的乙型肝炎患者血清标本分别采用实时荧光定量PCR检测HBV-DNA、时间分辨荧光免疫分析技术(TRFIA)定量检测HBV-e抗原和HBV PreS1抗原。结果PreS1抗原阳性率87.5%(460/526)高于HBV-DNA71.1%(374/526),有显著性差异(χ2=46.3,P<0.001),PreS1抗原阳性率高于HBeAg 37.1%(195/526),有显著性差异(χ2=251.6,P<0.001),HBV-DNA阳性率高于HBeAg(χ2=161.8,P<0.001),有显著性差异。结论TRFIA方法定量检测PreS1抗原,灵敏度高,特异性强,能够准确及时敏感地反映患者体内乙型肝炎病毒的复制情况。
Objective To compare the clinical value of PreS1, HBV-DNA and HBeAg in reflecting viral replication. Methods HBV-DNA was detected by real-time fluorescence quantitative PCR in serum samples of hepatitis B patients suspected of HBV replication, and quantitative detection of HBV-e antigen and HBV PreS1 antigen by time-resolved fluorescence immunoassay (TRFIA). Results The positive rate of PreS1 antigen was higher than that of HBeAg by 87.5% (460/526), which was significantly higher than that of HBV-DNA (374/526) (χ2 = 46.3, P <0.001) (Χ2 = 251.6, P <0.001). The positive rate of HBV-DNA was higher than that of HBeAg (χ2 = 161.8, P <0.001), with significant difference. Conclusion The TRFIA method can detect PreS1 antigen quantitatively and has high sensitivity and specificity. It can accurately and timely reflect the replication of hepatitis B virus in patients.