目的探讨ｎｍ２３基因表达在人肺癌中的作用。方法通过Ｎｏｒｔｈｅｒｎ印迹杂交，检测４０例人肺癌组织和１９例非癌肺组织的ｎｍ２３Ｈ１和ｎｍ２３Ｈ２ｍＲＮＡ表达，采用地高辛标记和检测系统显示杂交信号，并分析ｍＲＮＡ表达与肺癌临床特征的关系。结果低分化鳞癌的ｎｍ２３Ｈ２ｍＲＮＡ表达较中高分化鳞癌显著降低（Ｐ＜０．０１），小细胞肺癌的ｎｍ２３Ｈ１和ｎｍ２３Ｈ２ｍＲＮＡ表达较肺鳞癌明显降低。但在有或无淋巴结转移的原发癌灶组织间，以及肺癌的临床分期间，ｎｍ２３基因ｍＲＮＡ表达差异无显著性（Ｐ＞０．０５）。结论ｎｍ２３基因ｍＲＮＡ表达与肺癌的组织分化有关，未发现其在肺癌中的癌转移抑制作用。 Objective To investigate the role of nm23 gene expression in human lung cancer. Methods Northern blot hybridization was used to detect the expression of nm23H1 and nm23H2 mRNA in 40 cases of lung cancer and 19 cases of noncancer lung tissues. The signals were detected by digoxigenin labeling and detection system, and the mRNA expression and clinical features of lung cancer were analyzed. relationship. Results The expression of nm23H2 mRNA in poorly differentiated squamous cell carcinoma was significantly lower than that in moderately well differentiated squamous carcinoma (P<0.01). The expression of nm23H1 and nm23H2 mRNA in small cell lung cancer was significantly lower than that in lung squamous cell carcinoma. However, there was no significant difference in mRNA expression of nm23 in the primary tumor tissues with or without lymph node metastases and during the clinical course of lung cancer (P>0.05). Conclusions The mRNA expression of nm23 gene is related to the differentiation of lung cancer, and its metastasis suppressing effect in lung cancer was not found.