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目的探讨白藜芦醇(resveratrol,Res)对阿尔茨海默病(Alzheimer′s disease,AD)大鼠海马组织中小胶质细胞的增殖活化以及白细胞介素-1β(IL-1β)表达的影响。方法采用去卵巢合并D-半乳糖(100mg/kg)注射制备AD大鼠模型,60只雌性Wistar大鼠随机分为模型组、Res低、中、高剂量组(20、40、80mg/kg)、戊酸雌二醇组(0.8mg/kg)及假手术组(蒸馏水),连续灌胃12周,采用在体心脏灌流法,固定大鼠海马脑组织,利用免疫组化法分别检测大鼠海马组织小胶质细胞离子钙接头蛋白(Iba-1)以及IL-1β的表达。采用SPSS 16.0软件进行统计分析,免疫组化结果采用单因素方差分析,检验水准α=0.05。结果经单因素方差分析,6个组Iba-1的表达量总体均数差异有统计学意义[F(5,66)=30.632,P<0.01]。多重比较结果显示:模型组Iba-1表达量明显高于假手术组(P<0.05);Res低剂量组Iba-1表达量低于模型组(P<0.05);Res中、高剂量组以及戊酸雌二醇组Iba-1表达量低于模型组(P<0.01);Res中剂量组Iba-1表达量低于戊酸雌二醇组(P<0.01)。6个组IL-1β的表达量总体均数差异有统计学意义[F(5,66)=11.553,P<0.01]。多重比较结果显示:模型组IL-1β表达量高于假手术组(P<0.01);Res低、中、高剂量组以及戊酸雌二醇组IL-1β表达量低于模型组(P<0.01)。结论 Res可以抑制AD大鼠海马中过度增殖活化的小胶质细胞并且减少脑组织中IL-1β的表达,从而对AD大鼠神经系统起到保护作用。
Objective To investigate the effect of resveratrol (Res) on the proliferation and activation of microglia and the expression of interleukin-1β (IL-1β) in hippocampus of rats with Alzheimer’s disease (AD) . Methods AD rats were induced by ovariectomized with D-galactose (100 mg / kg). Sixty female Wistar rats were randomly divided into model group, Res low, medium and high dose groups (20, 40 and 80 mg / kg) , Estradiol valerate (0.8 mg / kg) and sham operation group (distilled water) for 12 weeks. The rats were perfused with the cardiomyocyte perfusion method and the hippocampal tissue was fixed by immunohistochemistry. Hippocampal microglia ionocalciferous adapter protein (Iba-1) and IL-1β expression. SPSS 16.0 software for statistical analysis, immunohistochemical results using one-way analysis of variance, test level α = 0.05. Results The single-factor analysis of variance showed that there was a significant difference in the total number of Iba-1 expression among the 6 groups (F (5,66) = 30.632, P <0.01]. The results of multiple comparisons showed that the expression of Iba-1 in model group was significantly higher than that in sham operation group (P <0.05); the expression of Iba-1 in Res low dose group was lower than that in model group (P <0.05) The expression of Iba-1 in estradiol valerate group was lower than that in model group (P <0.01). The expression level of Iba-1 in Res group was lower than that in estradiol valerate group (P <0.01). The total number of IL-1β expression in 6 groups showed statistically significant difference (F (5,66) = 11.553, P <0.01]. The results of multiple comparisons showed that the expression of IL-1β in model group was higher than that in sham operation group (P <0.01), and the expression of IL-1β in Res group was lower than that in model group (P < 0.01). Conclusion Res can inhibit the proliferation of activated microglia in the hippocampus and decrease the expression of IL-1β in the hippocampus of rats with Alzheimer’s disease, thereby protecting the nervous system of AD rats.