论文部分内容阅读
以中国南瓜(Cucurbita moschata)为试验材料,利用同源克隆和南瓜转录组unigene序列获得2个IMP基因的全长c DNA序列,将两个基因命名为Cm IMP1和Cm IMP2,Gen Bank登录号为KP735607和KP735608。Cm IMP1基因c DNA全长1 053 bp,包含1个810 bp的ORF,共编码269个氨基酸;Cm IMP2基因c DNA全长945 bp,包含1个807 bp的ORF,共编码268个氨基酸。序列分析发现两个基因均含有磷酸酶家族锂敏感的3个特殊结构域,系统进化树分析表明这2个南瓜Cm IMP与其他植物IMP有较高同源性(63.8%~94.0%),并与葫芦科作物最接近。采用荧光定量PCR研究Cm IMP在各组织及逆境条件下的表达模式显示,其表达具有组织特异性,在叶中表达最高,须和幼果次之;盐胁迫和干旱胁迫可强烈诱导Cm IMP表达,ABA对Cm IMP的诱导较微弱,推测Cm IMP在南瓜响应非生物胁迫的分子调控机制方面发挥重要作用。
The full length cDNA sequences of two IMP genes were obtained by homologous cloning and pumpkin transcriptome unigene sequences with Cucurbita moschata as test material. The two genes were named Cm IMP1 and Cm IMP2, and the Gen Bank accession number was KP735607 and KP735608. The cDNA of Cm IMP1 gene was 1 053 bp in length and contained a 810 bp ORF encoding 269 amino acids. The cDNA of Cm IMP2 gene was 945 bp in length and contained an ORF of 807 bp encoding a total of 268 amino acids. Sequence analysis revealed that the two genes contained three specific lithium-sensitive phosphatase families. Phylogenetic tree analysis showed that the two IMPs had high homology (63.8% -94.0%) with other plant IMP, The closest crops. Fluorescence quantitative PCR showed that expression of Cm IMP under various tissues and adversity conditions showed that the expression of Cm IMP was tissue-specific and highest in leaves, followed by young fruit. Salt stress and drought stress strongly induced expression of Cm IMP , ABA induced a weak induction of Cm IMP, suggesting that Cm IMP plays an important role in the molecular mechanism of pumpkin response to abiotic stress.