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为研究枇杷籽中的抗氧化与抗炎活性成分,将枇杷籽用70%乙醇提取获得粗提物,再依次用正己烷、乙酸乙酯、正丁醇、甲醇、蒸馏水提取得到不同极性的溶剂组分。采用福林酚法测定粗提物及各溶剂组分的总酚含量;通过DPPH和O2-自由基清除试验及β-胡罗卜素-亚油酸体系褪色抑制试验,评估粗提物及各组分的抗氧化活性;通过环氧合酶(COX)体外抑制试验,评价粗提物及各组分对COX-1和COX-2的抑制活性。结果表明:枇杷籽粗提物以水溶性组分为主(78.58%),水溶性组分的总酚含量和抗氧化活性高于粗提物,并显著高于其它溶剂组分(P<0.05)。枇杷籽粗提物、水溶性组分和甲醇组分具有COX-1和COX-2抑制活性,其活性排序为水溶组分>粗提物>甲醇组分,三者对COX-1和COX-2的抑制无明显的选择性。
In order to study antioxidant and anti-inflammatory active ingredients in loquat seed, the loquat seed was extracted with 70% ethanol to obtain the crude extract, and then with n-hexane, ethyl acetate, n-butanol, methanol, Solvent components. The total phenols content of the crude extract and each solvent component was determined by Folin phenol method. The DPPH and O2-free radical scavenging assay and the β-carotene-linoleic acid system discoloration inhibition test were used to evaluate the crude extract and The anti-oxidative activity of COX-1 and COX-2 were evaluated by in vitro inhibition of cyclooxygenase (COX). The results showed that the main components of the crude extracts of loquat seed were water-soluble components (78.58%), the total phenolic content and antioxidant activity of water-soluble components were higher than that of the crude extracts, and significantly higher than other solvent components (P <0.05 ). The crude extracts of loquat seed, water-soluble fraction and methanol fraction showed COX-1 and COX-2 inhibitory activity. The activities of the extracts were water-soluble fraction> crude extract> methanol fraction. 2 inhibition no obvious selectivity.