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目的研究血管内皮生长因子C(VEGF-C)和C-C家族趋化因子受体7(CCR7)与恶性黑色素瘤(恶黑)淋巴管浸润、淋巴结转移的关系及其预后价值。方法免疫组化方法检测56例恶黑组织中VEGF-C和CCR7的表达,淋巴管内皮细胞透明质酸受体1(LYVE-1)标记肿瘤的淋巴管,Kaplan-Meier法进行生存检验,应用Cox比例危险度模型筛选与恶黑预后有关的指标。结果肿瘤细胞胞浆中可检测到VEGF-C和CCR7的表达。CCR7表达与VEGF-C表达和淋巴管浸润有关,CCR7和VEGF-C协同增加淋巴管浸润,CCR7表达与淋巴结转移及预后无关。结论在恶黑组织中VEGF-C和CCR7诱导肿瘤细胞侵入到淋巴管,但CCR7和淋巴管浸润不能作为恶黑的预后指标。
Objective To investigate the relationship between the expression of vascular endothelial growth factor C (VEGF-C) and C-C family chemokine receptor 7 (CCR7) and lymphatic invasion and lymph node metastasis in malignant melanoma (malignant melanoma) and their prognostic value. Methods Immunohistochemistry was used to detect the expression of VEGF-C and CCR7 in 56 cases of malignant tissue. The lymphatic vessels of lymphatic endothelial cells were labeled with LYVE-1 and the Kaplan-Meier method was used for survival test. Cox proportional hazards model was screened for indicators related to malignant black prognosis. Results The expression of VEGF-C and CCR7 was detected in cytoplasm of tumor cells. The expression of CCR7 is related to the expression of VEGF-C and lymphangiogenesis, and CCR7 and VEGF-C synergistically increase lymphatic invasion. The expression of CCR7 has no relation with lymph node metastasis and prognosis. Conclusion VEGF-C and CCR7 induce invasion of tumor cells into lymphatic vessels in malignant tissue, but the infiltration of CCR7 and lymphatic vessels can not be used as prognostic indicators of malignant tumor.